The fusion gene constructs were generated using the Gateway multifragment recombination technique, as previously described (12 (link)). Briefly, fusion gene construction was based on PCR using sequence-verified open reading frames (ORFs) corresponding to each fusion gene fragment obtained from the ORFeome collaboration (www.orfeomecollaboration.org), Mammalian Gene Collection, and commercial ORF sources (Ultimate ORF Clones; Life Technologies) as PCR template. PCR primers were generated to amplify the desired fragments (left and right gene arms of each fusion) with terminal Gateway (Life Technologies) recombination sequences. The left and right arm PCR products were incorporated into pDONR vectors (Life Technologies) and pFUSE-B vector (Addgene, plasmid no. 97185), respectively, through BP recombination (Life Technologies), followed by multifragment recombination into the pFUSE-DEST_R1R4 vector (Addgene, plasmid no. 97186) through LR recombination reaction (Life Technologies) following the manufacturer’s recommendations. The reaction mixtures were incubated at room temperature overnight and subsequently transformed into STBL3 (Life Technologies) competent bacteria.