Western Blot Analysis of Cell Lysates
Corresponding Organization : Nanjing Drum Tower Hospital
Other organizations : Prince of Wales Hospital, Chinese University of Hong Kong, Second Military Medical University, Changhai Hospital
Variable analysis
- Cell lysates
- Proteins separated on SDS-PAGE gels
- Protein signals generated by enhanced chemiluminescence
- SDS-PAGE gel concentration (6-12%)
- Blocking buffer (TBST with 5% nonfat milk or bovine serum albumin)
- Incubation time for blocking (2 hours at room temperature)
- Incubation time for primary antibodies (overnight at 44 °C)
- Dilution of secondary antibodies (1:5000)
- Positive control: Not explicitly mentioned
- Negative control: Not explicitly mentioned
Annotations
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