Immunofluorescence Microscopy Technique

Immunofluorescence was performed as previously described (Oegema et al., 2001 (link)), with slides fixed in methanol for 40–45 min. The following antibodies were used: rabbit anti-AIR-2 (1:500; gift from Jill Schumacher), rabbit anti-BUB-1 (1:2800; gift from A Desai), rabbit anti- DHC-1 (1:100, gift from P Gönczy), rabbit anti-GFP (1:500; AbCam, Cambridge, UK), mouse anti-GFP (1:500; AbCam), rabbit anti-KLP-18 (1:10,000, gift of O Bossinger), rabbit anti-KNL-3 (1:3800; gift from A Desai), mouse anti-MPM-2 (1:500; AbCam and Millipore, Billerica, MA), rabbit anti-SEP-1 (1:200; gift from A Golden), mouse anti-α-tubulin-FITC (1:500; Sigma). Alexa-flour-conjugated secondary antibodies (Invitrogen, Carlsbad, CA) were used at 1:500.

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Muscat C.C., Torre-Santiago K.M., Tran M.V., Powers J.A, & Wignall S.M. (2015). Kinetochore-independent chromosome segregation driven by lateral microtubule bundles. eLife, 4, e06462.

Publication 2015

rabbit anti-GFP mouse anti-GFP Alexa-flour-conjugated secondary antibodies

Antibodies Fitc Flour Immunofluorescence Methanol Mouse Rabbit α tubulin

Corresponding Organization :

Other organizations : Northwestern University, Imaging Center, Indiana University Bloomington

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