OVA-Specific T Cell Analysis

OVA_257–264 (SIINFEKL) peptide was purchased from the American Peptide Company (Sunnyvale, CA, USA). PE, PE/Cy7, PerCP, PerCP/Cy5.5, or APC conjugated Thy1.2 (Clone 53-2.1), TCRVβ5 (MR9-4), TCRVα2 TCR (B20.1), CD44 (Clone IM7), IFN-γ (Clone XMG1.2) and IL-2 (JES6-5H4) were purchased from Biolegend (San Diego, CA, USA). Mouse α-Notch2 Ab was kindly provided by Dr. Hideo Yagita (Juntendo University School of Medicine, Japan) [13 (link),14 (link)].

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Xiong X., Das J.K., Song J., Ni B., Ren X., Yang J.M, & Song J. (2018). Protective Cancer Vaccine Using Genetically Modified Hematopoietic Stem Cells. Vaccines, 6(3), 40.

Publication 2018

PerCP/Cy5.5 IL-2 (JES6-5H4)

Cd44 Clone Cy5 5 Ifn γ Mouse Notch2 Peptide

Corresponding Organization : Texas A&M Health Science Center

Other organizations : Army Medical University, Pennsylvania State University

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Variable analysis

independent variables

OVA_257–264 (SIINFEKL) peptide

dependent variables

Expression of Thy1.2 (Clone 53-2.1), TCRVβ5 (MR9-4), TCRVα2 TCR (B20.1), CD44 (Clone IM7), IFN-γ (Clone XMG1.2) and IL-2 (JES6-5H4)

control variables

Mouse α-Notch2 Ab was kindly provided by Dr. Hideo Yagita (Juntendo University School of Medicine, Japan)

controls

Positive control: Not explicitly mentioned.
Negative control: Not explicitly mentioned.

Annotations

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