Protocol detail

Culturing Human Head and Neck Cancer Cells

Find Similar Protocols

Human HNSCC lines CAL27 and SCC15 were purchased from ATCC (Manassas, VA). The cells were maintained in complete Dulbecco's Modified Eagle's Medium (DMEM) containing 10% fetal bovine serum (FBS, Invitrogen, Carlsbad, CA), 100 units of penicillin and 100 μg of streptomycin at 37°C in 5% CO₂ as described [43 (link)–45 (link)], Unless indicated otherwise, all chemicals were purchased from Sigma-Aldrich (St. Louis, MO) or Thermo Fisher Scientific (Waltham, MA).

Free full text: Click here

Zhang F., Li Y., Zhang H., Huang E., Gao L., Luo W., Wei Q., Fan J., Song D., Liao J., Zou Y., Liu F., Liu J., Huang J., Guo D., Ma C., Hu X., Li L., Qu X., Chen L., Yu X., Zhang Z., Wu T., Luu H.H., Haydon R.C., Song J., He T.C, & Ji P. (2017). Anthelmintic mebendazole enhances cisplatin's effect on suppressing cell proliferation and promotes differentiation of head and neck squamous cell carcinoma (HNSCC). Oncotarget, 8(8), 12968-12982.

Publication 2017

CAL27 SCC15 FBS streptomycin

Cells Hnscc Human Penicillin Streptomycin

Top 5 similar protocols

Protocol cited in 2 other protocols

Variable analysis

independent variables

Cell lines (CAL27 and SCC15)

dependent variables

Not explicitly mentioned

control variables

Complete Dulbecco's Modified Eagle's Medium (DMEM) containing 10% fetal bovine serum (FBS, Invitrogen, Carlsbad, CA), 100 units of penicillin and 100 μg of streptomycin
Temperature (37°C)
CO2 concentration (5%)

controls

Positive control: not specified
Negative control: not specified

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!