Human Hepatocyte Isolation and Culture

Human hepatocytes were isolated from histologically normal liver and were provided by D. Geller (Department of Surgery, University of Pittsburgh, Pittsburgh, PA) according to a protocol approved by the Institutional Review Board. Human hepatocytes were prepared by a three-step collagenase perfusion technique, modified as described previously (43 (link)). Isolated human hepatocytes were cultured in William’s medium E supplemented with 5% calf serum (GE Healthcare Life Sciences), penicillin (100 U/ml), streptomycin (100 U/ml), 2 mM L-glutamine, and 15 mM Hepes.

Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link: Access Free Full Text.

Deng M., Loughran P.A., Zhang L., Scott M.J, & Billiar T.R. (2015). Shedding of the tumor necrosis factor (TNF) receptor from the surface of hepatocytes during sepsis limits inflammation through cGMP signaling. Science signaling, 8(361), ra11.

Publication 2015

calf serum penicillin

Collagenase Cultured medium Glutamine Hepatocytes Hepes Human Institutional review board Liver Penicillin Perfusion Serum Streptomycin Surgery

Corresponding Organization : University of Pittsburgh

Top 5 similar protocols

Variable analysis

independent variables

Isolation protocol for human hepatocytes

dependent variables

Viability and function of isolated human hepatocytes

control variables

Histologically normal liver tissue used as source of human hepatocytes
Culture conditions: William's medium E supplemented with 5% calf serum, penicillin (100 U/ml), streptomycin (100 U/ml), 2 mM L-glutamine, and 15 mM Hepes

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!