Western blot was performed according to a previously described method [32 (link)] with antibodies for anti-human KLF17 (1:200, Santa Cruz, CA; 1:200, Sigma, CA), anti-uPA (1:200, Sigma, CA), anti-Phospho-p38/MAPK, anti-Phospho-pERK1/2, anti-Phospho-pSAPK/JNK, anti-Phospho-Akt, anti-Phospho-p44/42/MAPK, anti- Phospho-PI3-Kinasep85 and anti-Phospho-Src (Tyr527) (Cell Signaling Tech., Danvers, MA). Anti-GAPDH (Bioworld Tech, Inc., Nanjing, China) was used to detect the internal control.
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