Antibody staining was performed by standard procedures for imaginal discs [66 (link)]. Rabbit anti-Cleaved Caspase-3 (1:400, Cell Signaling Technology, CST, Cat # 9661, Danvers, MA, USA) was used as a primary antibody, and goat anti-rabbit CY3 (1:1000, Life technologies, Cat # A10520) was used as a secondary antibody.
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