Transfection of Cell Lines with FOXM1

All cells were transfected using jetPRIME reagent (Cat. 114-15, Polyplus Transfection, Inc., NY, USA) at a ratio of DNA to reagent of 1:3 according to the manufacturer’s protocol. In our previous study, the pcDNA6-V5-His-tag expression vector was purchased from Invitrogen (Cat. V22020, Carlsbad, CA) and pFOXM1 was provided by Dr. Ju-Seog Lee (MD Anderson Cancer Centre, TX, USA) [32 (link)]. Scrambled RNA (scRNA) was purchased from GenePharma (Shanghai Co., Ltd., China), and siFOXM1 was synthesized at the ST Pharm Oligo center (ST Pharm, Seoul, Korea).

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Kim S.K., Ahn S.G., Mun J.Y., Jeong M.S., Bae S.J., Lee J.S., Jeong J., Leem S.H, & Chu I.S. (2020). Genomic Signature of the Standardized Uptake Value in 18F-Fluorodeoxyglucose Positron Emission Tomography in Breast Cancer. Cancers, 12(2), 497.

Publication 2020

jetPRIME reagent Scrambled RNA (scRNA)

Cancer Cells Oligo Transfection Vector

Corresponding Organization : Korea University of Science and Technology

Other organizations : The University of Texas MD Anderson Cancer Center

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Variable analysis

independent variables

Transfection with pcDNA6-V5-His-tag expression vector
Transfection with pFOXM1
Transfection with scrambled RNA (scRNA)
Transfection with siFOXM1

dependent variables

Not explicitly mentioned

control variables

Transfection reagent (jetPRIME) used at a ratio of DNA to reagent of 1:3 according to the manufacturer's protocol

controls

Positive control: pcDNA6-V5-His-tag expression vector
Negative control: scrambled RNA (scRNA)

Annotations

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