Total RNA Extraction and RT-qPCR Analysis
Corresponding Organization : Central South University
Other organizations : The University of Texas Health Science Center at San Antonio, The University of Texas Health Science Center at Houston
Variable analysis
- None explicitly mentioned
- Gene expression levels measured by RT-qPCR
- RNA isolation method (RNA Express Total RNA Kit)
- Reverse transcription method (RevertAid First Strand cDNA Synthesis Kit)
- RT-qPCR instrument (StepOne Plus)
- RT-qPCR master mix (SYBR Green qPCR Master Mix (2×))
- Primer sequences (listed in Table 1)
- None explicitly mentioned
Annotations
Based on most similar protocols
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!