Total cellular RNA was isolated with RNA Express Total RNA Kit (M050, NCM Biotech, China) (37 (link)). The RNA was reverse transcribed using the RevertAid First Strand cDNA Synthesis Kit (Thermo). We performed RT-qPCR on the StepOne Plus (Applied Biosystems, United States) by utilizing SYBR Green qPCR Master Mix (2×) (Bimake, United States). The primers are listed in Table 1.
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