Lipid-based Nanoparticle Formulation and Evaluation

Soybean phosphatidylcholine (SPC), cholesterol, and DSPE-PEG2000 were purchased from Avanti lipids (Avanti Polar Lipids, Alabaster, AL, USA). Dimethyl sulfoxide (DMSO), copper chloride (CuCl₂), and 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Fetal bovine serum (FBS) and DMEM-F12 medium (access date 12 March 2021,Gibco, Grand Island, NY, USA) were purchased from Thermo Fisher Scientific (Waltham, MA, USA), respectively. DQ was synthesized in our laboratory based on a previous report [12 (link)].

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Kang X., Wang Q., Wu S., Wang C., Annaji M., Huang C.H., Shen J., Chen P, & Babu R.J. (2023). Liposomal DQ in Combination with Copper Inhibits ARID1A Mutant Ovarian Cancer Growth. Biomolecules, 13(5), 744.

Publication 2023

DSPE-PEG2000 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) Fetal bovine serum (FBS) DMEM-F12 medium

Alabaster Bromide Chloride Cholesterol Copper Cucl2 Dimethyl sulfoxide Dspe peg2000 Fetal bovine serum Lipids Phosphatidylcholine Soybean

Corresponding Organization : Auburn University

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Variable analysis

independent variables

Cholesterol
DSPE-PEG2000

dependent variables

Cell viability (measured by MTT assay)

control variables

CuCl2
DMEM-F12 medium
Fetal bovine serum (FBS)

controls

Positive control: Not specified.
Negative control: Not specified.

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