Kidney tissues were embedded in paraffin and 2-μm kidney sections for periodic acid-Schiff (PAS) staining as described (40 (link), 42 (link)). Representative images of kidney sections (cortex and outer medulla) are shown to illustrate tubular injury that displayed cast formation and tubular dilation. Injured tubular index was scored by the percentage of tubules in the corticomedullary junction that displayed cell necrosis, loss of brush border, cast formation, edema, and tubular dilation as follows: 0, none; 1, ≤10%; 2, 11–25%; 3, 26–45%; 4, 46–75%; 5, >76%. For immunostaining, we used biotinylated L. tetragonolobus lectin stain (Vector Labs), Tamm-Horsfall protein (THP) stain (Santa Cruz Biotechnology), anti-mouse IRF8 (Abcam), rabbit anti-mouse CD3 (Abcam), rat anti-mouse Ly6B.2 (Serotec, UK), and rat anti-mouse MHCII (I-A/I-E) (eBioscience™) (Table S2). All results were quantified by Image J software. To count interstitial cells, 10 cortical high-power fields (HPF) (400×). All assessments were performed by two blinded observers (CL and CS).
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