Tobacco Gene Expression Profiling

Total RNA from tobacco roots, stems, leaves, floral tissues including sepals, petals, stamens and pistils were prepared using TRIzol Reagent (Invitrogen) and first-strand cDNAs were generated using the cDNA EcoDry kit (Clontech, Madison, USA). The qPCR conditions and gene-specific primers are described in previous studies [20 (link),30 (link)]. Gene-specific primers for NtF3′5′H, NtMYB3 and NtETC1 are listed in Table S1. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) gene, which was previously used in gene expression analysis of transgenic tobaccos was used as an internal reference [8 (link),30 (link)]. Three biological replicates were examined for each sample.

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Kim D.H., Park S., Lee J.Y., Ha S.H, & Lim S.H. (2018). Enhancing Flower Color through Simultaneous Expression of the B-peru and mPAP1 Transcription Factors under Control of a Flower-Specific Promoter. International Journal of Molecular Sciences, 19(1), 309.

Publication 2018

TRIzol Reagent EcoDry kit

Biological Cdna Gene Gene expression analysis Glyceraldehyde 3 phosphate dehydrogenase Ntf3 Pistils Primers Roots Stems Tissues Tobacco Transgenic Trizol

Corresponding Organization : Rural Development Administration

Other organizations : Kyung Hee University

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Variable analysis

independent variables

Tissue type (tobacco roots, stems, leaves, floral tissues including sepals, petals, stamens and pistils)

dependent variables

Gene expression levels of NtF3'5'H, NtMYB3 and NtETC1

control variables

GAPDH gene expression (used as an internal reference)

controls

Three biological replicates were examined for each sample

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