BLAST searches were performed using NCBI-BLAST 2.3.0 with an E-value threshold of 0.001, 20 threads and unlimited number of hits. The whole set of protein sequences in eggNOG v4.5 was used as target database (http://eggnogdb.embl.de/download/eggnog_4.5/eggnog4.proteins.core_periphery.fa.gz ). While annotating query sequences, self hits were excluded both from BLAST and eggNOG-mapper hits to avoid circular annotations. No taxonomic restrictions were applied when transferring GO terms from BLAST or eggNOG-mapper hits (automatic taxonomic adjustment was manually disabled). eggNOG-mapper was called with the following parameters: “ – m hmmer – - tax_scope NOG - –target_orthologs all – - go_evidence experimental – - excluded_taxa [self_taxid] - –cpu 20 ” . The same eggNOG v4.5 Gene Ontology annotations were used both for BLAST and eggNOG-mapper.
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