Comparative Analysis of BMAT in C3H and B6 Mice

The Institutional Animal Care and Use Committee (IACUC) at Washington University in St. Louis approved all procedures, and these experiments were performed in AAALAC accredited facilities. For all experiments, C3H/HeJ (C3H, Stock:000659) and C57BL/6J (B6, Stock:000664) mice were obtained from Jackson Labs; mice were acclimatized for 1 week prior to experiments. Mice were housed on a 12-h light/dark cycle at 70 ± 2 degrees Fahrenheit and fed standard chow (LabDiet® 5053). Relative to B6 mice, C3H mice are known to have a significant expansion of BMAT in the proximal tibia by 12-weeks of age (29 (link)).