Mitochondrial Dysfunction Evaluation

To assess mitochondrial damage, using flow cytometry by co-staining MitoTracker Deep Red (Thermo Fisher Scientific) and MitoTracker Green (Thermo Fisher Scientific) as previously described^{29 (link)}. Mitochondrial membrane potential was determined by a JC-1 staining kit (Sigma Aldrich) following the manufacturer’s instruction as previously described^{13 (link)}.

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Jiang T., Liu E., Li Z., Yan C., Zhang X., Guan J., Zhan Y., Zhao B, & Ding W. (2024). SIRT1-Rab7 axis attenuates NLRP3 and STING activation through late endosomal-dependent mitophagy during sepsis-induced acute lung injury. International Journal of Surgery (London, England), 110(5), 2649-2668.

Publication 2024

MitoTracker Deep Red MitoTracker Green JC-1 staining kit

Corresponding Organization :

Other organizations : Harbin Medical University, Second Affiliated Hospital of Harbin Medical University, Northeast Forestry University

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Mitochondrial damage
Mitochondrial membrane potential

control variables

None explicitly mentioned

controls

Positive control: Not specified
Negative control: Not specified

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