Tissue samples were maintained in cold saline and brought to the laboratory within 2–4 h of procurement from the donor. Samples were rapidly processed using enzymatic and mechanical digestion to obtain lymphocyte populations with high viability as described in detail (Sathaliyawala et al., 2013 (link); Thome et al., 2014 (link), 2016 (link)). Lymphocytes were isolated from blood using lymphocyte separation media (CellGro; CellGenix) and ACK lysis buffer as previously described (Sathaliyawala et al., 2013 (link)). Lymphocytes were either analyzed immediately or cryopreserved for future analysis.
Protocol detail
Isolation and Preservation of Lymphocytes
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Corresponding Organization : Columbia University Irving Medical Center
Other organizations : University of Arizona
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Variable analysis
independent variables
- Enzymatic and mechanical digestion to obtain lymphocyte populations
- Lymphocyte viability
- Tissue samples maintained in cold saline
- Tissue samples brought to the laboratory within 2–4 h of procurement from the donor
- Lymphocytes isolated from blood using lymphocyte separation media and ACK lysis buffer
- Positive control: Not explicitly mentioned
- Negative control: Not explicitly mentioned
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