Lung tissues were fixed in 10% buffered formalin solution (pH7.2) for overnight, and serially sectioned to 15 μm at 20ºC. Immunohistochemistry was performed as described.33 (link) Immunoreactivity scores were analyzed using Image Scope V 10.0 software from Aperio Technologies (Vista, CA). The size and number of the metastases were also quantified and counted using Image Scope V 10.0 software (Aperio Technologies, Vista, CA).
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Quantitative Analysis of Lung Metastases
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Corresponding Organization : National Cancer Institute
Other organizations : Southern Medical University, Nanfang Hospital
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Variable analysis
independent variables
- Fixation of lung tissues in 10% buffered formalin solution (pH7.2) for overnight
- Immunoreactivity scores analyzed using Image Scope V 10.0 software
- Size and number of the metastases quantified and counted using Image Scope V 10.0 software
- Temperature of sectioning at 20ºC
- Positive control: Not explicitly mentioned
- Negative control: Not explicitly mentioned
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