The cardiovascular drugs verapamil hydrochloride, diltiazem hydrochloride, nifedipine, amlodipine besylate, amiodarone hydrochloride, quinidine, propafenone, valsartan, irbesartan, benazepril hydrochloride, and captopril were all purchased from Aladdin (Shanghai, China). The vonoprazan was obtained from Shanghai Send Pharm, and its carboxylic acid metabolite, MI, was provided by Wuxi Apptec Co., Ltd. (Shanghai, China).
Diazepam from J&K Scientific Ltd. (Beijing, China) was selected as the internal standard (IS). Nicotinamide adenine dinucleotide phosphate (NADPH) was purchased from Coolaber (Beijing, China). Methanol and acetonitrile of high-performance liquid chromatography (HPLC) grade were obtained from Merck Company (Darmstadt, Germany). Formic acid was purchased from Sigma-Aldrich (St. Louis, United States). Ultra-pure water was produced by the reagent-standard water purification system Milli-Q (Millipore, United States). The RLMs were prepared according to our previously reported method (Wang et al., 2020 (link)), and the HLMs were purchased from Bioruler (Wuhan, China).
Diazepam from J&K Scientific Ltd. (Beijing, China) was selected as the internal standard (IS). Nicotinamide adenine dinucleotide phosphate (NADPH) was purchased from Coolaber (Beijing, China). Methanol and acetonitrile of high-performance liquid chromatography (HPLC) grade were obtained from Merck Company (Darmstadt, Germany). Formic acid was purchased from Sigma-Aldrich (St. Louis, United States). Ultra-pure water was produced by the reagent-standard water purification system Milli-Q (Millipore, United States). The RLMs were prepared according to our previously reported method (Wang et al., 2020 (link)), and the HLMs were purchased from Bioruler (Wuhan, China).
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