RNA Extraction and miRNA Expression Profiling

As previously described [17 (link), 21 (link)], total RNA from chondrocytes and cartilage tissues was extracted using TRIzol (CW Biotech, Beijing, China). RT-qPCR was performed after reverse transcription as per the protocol provided with the miRNA reverse transcription kit (TaKaRa, Japan). β-Actin levels were used as the internal reference. Relative miRNA expression was calculated using the 2^−ΔΔCt method. Table 1 lists the sequences for the used.

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Yuan Y., Liu Q., Wu Z, & Luo W. (2020). Mechanistic Insight on the Interaction between OPN and Integrin ανβ3 in Osteoarthritis. BioMed Research International, 2020, 2905634.

Publication 2020

TRIzol miRNA reverse transcription kit

Actin Cartilage tissues Chondrocytes Mirna Reverse transcription Trizol

Corresponding Organization : Xiangya Hospital Central South University

Other organizations : Third Xiangya Hospital

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Variable analysis

independent variables

Not explicitly mentioned

dependent variables

MiRNA expression

control variables

β-Actin levels

controls

Positive control: Not mentioned
Negative control: Not mentioned

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