Genetically Engineered Mouse Models

BALB/c (H-2K^d) and C57BL/6 (H-2K^b) mice were purchased from Janvier Labs (Le Genest-Saint-Isle, France). Luciferase-transgenic C57BL/6 mice (H-2K^b) were bred in the animal facility of the Center for Clinical Research at the Medical Center – University of Freiburg, Germany. Mice carrying a floxed Xbp1 allele (Xbp1^fl/fl) have been previously described^{17 (link)} and were a kind gift from Dr. Laurie Glimcher, Cornell University, USA. B6. Cg-Tg(Vil^1-cre)997Gum/J mice expressing the Cre recombinase in villus and crypt intestinal epithelial cells (Vil^Cre) were purchased from the Jackson Laboratory. Vil^CreXbp1^fl/fl mice and Xbp1^fl/fl littermates were generated by crossing these two strains. All animals were housed under specific pathogen-free conditions at the animal facility of the Center for Clinical Research (ZKF, Freiburg, Germany). Genotypes were confirmed via polymerase chain reaction (PCR). All animal protocols (G-16/018, G-17/063, G-18/063; X-13/07J; X-15/10A; X20/06K) were approved by the Federal Ministry for Nature, Environment and Consumer Protection of the state of Baden-Württemberg, Germany.
All other methods are provided in the Online Supplementary Appendix.