OvCa cells were transfected14 (link) with 30 nM pre-miR-193b, scrambled control oligo (Ambion), 30 nM miRCURY LNA anti-miR-193b, or scrambled control LNA oligo (Exiqon) using siPORTNeoFX (Ambion). The cells were used for experiments 48 h after transfection or as indicated.
Protocol detail
miR-193b Regulation in OvCa Cells
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Corresponding Organization : University of Chicago
Other organizations : Indiana University Bloomington, Northwestern University
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Variable analysis
independent variables
- 30 nM pre-miR-193b
- Scrambled control oligo (Ambion)
- 30 nM miRCURY LNA anti-miR-193b
- Scrambled control LNA oligo (Exiqon)
- Not explicitly mentioned
- SiPORTNeoFX (Ambion)
- Time after transfection (48 h or as indicated)
- Scrambled control oligo (Ambion)
- Scrambled control LNA oligo (Exiqon)
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