Cell Culture Conditions for Various Cell Lines

HeLa cells were cultured in RPMI-1640
(Gibco BRL) supplemented with 10% neonatal bovine serum (NBS) (Sijiqing
Biotech, Hangzhou, China). 4T1, H1975, and KB cells were incubated
in RPMI-1640 (Gibco BRL) containing 10% fetal bovine serum (Gibco
BRL). RG, HK-2, and MCF-7 cells were grown in DMEM (Gibco BRL) containing
10% fetal bovine serum (Gibco BRL). All cell lines were maintained
in a 5% CO₂-humidified atmosphere at 37 °C.

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Chang L., Wu X., Ran K., Tian Y., Ouyang X., Liu H., Gou S., Zhang Y, & Ni J. (2023). One New Acid-Activated Hybrid Anticancer Peptide by Coupling with a Desirable pH-Sensitive Anionic Partner Peptide. ACS Omega, 8(8), 7536-7545.

Publication 2023

RPMI-1640 fetal bovine serum DMEM

Atmosphere Bovine Cell lines Fetal bovine serum Hela cells Kb cells Mcf 7 cells Neonatal Serum

Corresponding Organization : Chinese Academy of Medical Sciences & Peking Union Medical College

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Variable analysis

independent variables

Cell lines used (HeLa, 4T1, H1975, KB, RG, HK-2, MCF-7)

dependent variables

Cell growth and proliferation

control variables

Culture medium (RPMI-1640 and DMEM)
Serum supplements (10% neonatal bovine serum and 10% fetal bovine serum)
Incubation conditions (5% CO2-humidified atmosphere at 37 °C)

controls

No positive or negative controls explicitly mentioned

Annotations

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