Genetically Modified Mice for Immunology

All mice were on a C57Bl/6 background. Itk^−/−, TCRδ (γδ T cell-deficient mice, B6.129P2-Tcrd^tm1Mom/J, Jax Labs), IL4Rα^−/− (a kind gift from Dr. Frank Brombacher, University of Cape Town, South Africa, via Dr. Fred Finkelman, University of Cincinnati^{51 (link)}). IL17A-GFP reporter mice (C57BL/6-Il17a^tm1Bcgen/J were purchased from Biocytogen, and Foxp3-RFP reporter mice (C57BL/6-Foxp3^tm1Flv/J) were from the Jackson Laboratory (Bar Harbor, ME). Reporter strains were crossed to generate IL17A-GFP/Foxp3-RFP dual reporter strains in WT or Itk^−/− background^{52 (link)}. TCRδ/Itk^−/− were previously described ^{53 (link)}. All mice were housed in a specific pathogen-free environment, and male and female mice were between 6 and 8 weeks of age when used. All experiments were approved by the Office of Research Protection’s Institutional Animal Care and Use Committee at The Pennsylvania State University and Cornell University.