IHC for brachyury was reviewed in all cases. IHC for SMARCB1 was performed on 4-µm-whole sections of formalin-fixed, paraffin embedded tissue blocks. The primary antibody used was a mouse monoclonal anti-BAF47 antibody (1:30; BD bioscience, San Jose, CA). Antigen retrieval, antibody incubation, and chromogen counterstaining were performed in a BenchMark ULTRA automated immunostainer (Ventana, Tucson, AZ) as previously described.10 (link)
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Immunohistochemical Analysis of Brachyury and SMARCB1
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Corresponding Organization : Memorial Sloan Kettering Cancer Center
Other organizations : University of New England
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Variable analysis
independent variables
- Antibody used (mouse monoclonal anti-BAF47 antibody)
- SMARCB1 expression
- Formalin-fixed, paraffin embedded tissue blocks
- 4-µm-whole sections
- Antigen retrieval, antibody incubation, and chromogen counterstaining performed in a BenchMark ULTRA automated immunostainer
- Positive control: Not explicitly mentioned
- Negative control: Not explicitly mentioned
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