Sm22αCre, Brg1F/F,
Mef2cCre, R26R and
Tnnt2-rtTA;Tre-Cre mice have been described23 (link), 24 (link),
27 (link), 31 (link), 38 (link). Immunostaining, RNA in
situ hybridization, quantitative RT-PCR, and whole embryo culture were performed as
described23 (link), 26 (link). TAC was modified from previous descriptions20 (link). The pressure load caused by TAC was
verified by the pressure gradient across the aortic constriction measured by
echocardiography. Only mice with a pressure gradient > 30 mmHg were analyzed
for cardiac hypertrophy and gene expression. Curve modeling was performed with the
Levenburg-Marquardt non-linear regression method and XLfit software. Detailed
methods can be found in theSupplementary Information .
Mef2cCre, R26R and
Tnnt2-rtTA;Tre-Cre mice have been described23 (link), 24 (link),
27 (link), 31 (link), 38 (link). Immunostaining, RNA in
situ hybridization, quantitative RT-PCR, and whole embryo culture were performed as
described23 (link), 26 (link). TAC was modified from previous descriptions20 (link). The pressure load caused by TAC was
verified by the pressure gradient across the aortic constriction measured by
echocardiography. Only mice with a pressure gradient > 30 mmHg were analyzed
for cardiac hypertrophy and gene expression. Curve modeling was performed with the
Levenburg-Marquardt non-linear regression method and XLfit software. Detailed
methods can be found in the
