Modulation of Legume-Rhizobia Symbiosis by Gibberellin

For Figs 1D, 5A, and B, pea seeds were nicked and treated at the time of planting with either 5 µl of ethanol (control) or 10 µg of GA₃ (Merck, Australia) in 5 µl of ethanol. Seeds were planted and inoculated at 7 d as described by Foo and Davies (2011) (link). At 25 d, 5 µl of ethanol (control) or GA₃ in 5 µl of ethanol was applied to the uppermost mature leaf. For Fig. 1D, roots were stained for lacZ and infection structures recorded as described above. For Fig. 5A and B, nodule number and size were recorded as described by Foo et al. (2016b (link)) at 37 d. For Fig. 5C and D, lupin seeds were nicked and treated at the time of planting with either 10 µl of ethanol (control), 10 µg of GA₃, and/or 10 µg of paclobutrazol (PAC; Sigma Aldrich Pty. Ltd, Australia) in 10 µl of ethanol, planted and inoculated with appropriate bacteria as described by Foo et al., (2016a (link)), and nodule size was recorded at 28 d as described by Foo et al. (2016b (link)).