EtOH, Sucrose and sodium saccharin were used as drinking solutions. EtOH (99.8%) solutions (PanReac AppliChem, Barcelona, Spain) diluted to 5, 10, 20, 30 or 40% (v/v) were prepared in sterilized tap water. Sucrose solutions were prepared by dissolving ≥ 99.5% Sucrose (Sigma-Aldrich Química S.A. Madrid, Spain) in sterilized tap water to 5, 10, 20 or 40% (w/v). Sodium saccharine (Sigma-Aldrich, ≥ 98%) solutions at 0.13, 0.26, 0.53 or 1.06% (w/v) were dissolved in sterilized tap water. Concentrations of all drinking solutions were based on previous studies (Rhodes et al., 2005 (link), 2007 (link); Kamdar et al., 2007 ; Pastor et al., 2011 (link); Tarragón et al., 2012 (link)) as well as on our own pilot studies. In the case of EtOH, a direct study of the influence of availability of different concentrations and number of tubes was included in the present study (Experiment 1). NTX hydrochloride (Sigma-Aldrich) was diluted in 0.9% physiological saline and administered intraperitoneally (IP; 10 mL/kg injection volume). The dose range of NTX (4, 8 or 16 mg/kg) used in the present study was based on previous work (Kamdar et al., 2007 ; Tarragón et al., 2012 (link); Fultz et al., 2017 (link)).
Protocol detail
Alcohol, Sweeteners Consumption Study
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Corresponding Organization : Universitat Jaume I
Other organizations : University of Michigan–Ann Arbor
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Variable analysis
independent variables
- EtOH (ethanol) concentrations (5%, 10%, 20%, 30%, 40% v/v)
- Sucrose concentrations (5%, 10%, 20%, 40% w/v)
- Sodium saccharin concentrations (0.13%, 0.26%, 0.53%, 1.06% w/v)
- Naltrexone (NTX) doses (4 mg/kg, 8 mg/kg, 16 mg/kg)
- Drinking behavior (not explicitly mentioned)
- Sterilized tap water used to prepare all drinking solutions
- Physiological saline used to dilute naltrexone (NTX)
- Not explicitly mentioned
- Not explicitly mentioned
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