Anti-Ly6G depleting antibody clone 1A8 or isotype control 2A3 (BioXcell, West Lebanon, NH ) was administered as described by Nandi et al50 (link). Ly6GPos cell depletion was verified by FACS analysis of CD11bHigh GR1Pos Ly6CNeg cell populations (Supplementary Fig. 2a ).
Protocol detail
Ly6G+ Cell Depletion Protocol
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Anti antibody
Cell
Clone
Isotype
Populations
Corresponding Organization :
Other organizations : University of Massachusetts Chan Medical School, Albany Medical Center Hospital
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Variable analysis
independent variables
- Anti-Ly6G depleting antibody clone 1A8
- Isotype control 2A3
- Ly6G^Pos cell depletion
- Administration of antibodies as described by Nandi et al.
- FACS analysis of CD11b^High GR1^Pos Ly6C^Neg cell populations
- Positive control: Anti-Ly6G depleting antibody clone 1A8
- Negative control: Isotype control 2A3
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