Cell Line Characterization and Cultivation

The mouse melanoma B16F10 (ATCC code CRL-6475) and the human melanoma SK-Mel-28 (ATCC code HTB-72) cell lines were obtained from the American Type Cell Culture Collection (ATCC, Manassas, VA, USA). Primary melanoma culture VM-21 and VM48 were obtained in Vienna and are characterised in the referenced publications.^{45 (link),46 (link)} The ovarian cancer cell model A2780 together with its cisplatin-resistant subline A2780/cis were obtained from Sigma-Aldrich. The colon cancer cell line HCT116 was obtained from ATCC (American Type Culture Collection). The oxaliplatin-resistant subline was established at the Institute of Cancer Research, Medical University Vienna, as published.^{31 (link)} All cell lines, except the HCT116 model, were cultured at 37 °C and 5% CO₂ in RPMI 1640 supplemented with 10% heat-inactivated fetal bovine serum and 2% l-glutamine (0.6 mg mL⁻¹). HCT116 was grown in ATCC-formulated McCoy’s 5a Medium again with 10% heat-inactivated fetal bovine serum. For ATCC cell lines, the medium was supplemented with antibiotics (2% de penicillin/streptomycin (200 IU ml⁻¹ and 200 μg mL⁻¹) and 0.1 mg mL⁻¹ gentamicin).