Protocol detail

Antibodies Used in Cell Biology Study

Find Similar Protocols

The following primary antibodies were used in this study: Rabbit anti-dsRed (Clontech #632496), Rat anti-E Cadherin (Life Technologies #13–1900), Rabbit anti-Fat3 and polyclonal Mouse anti-Fat3 (Lisa Goodrich, Harvard Medical School [18 (link)]), Goat anti-GFP (Abcam #5450, IF and WB), Goat anti-GFP (Abcam #6673, IP only), Rabbit anti-GST (Cell Signaling Technology #2625S) Mouse anti-HA (Covance #MMS-101P), Mouse anti-Kif1A (BD Biosciences #612094), Rabbit anti-Kif5B (Thermo # PA1-642), Goat anti-Kif5B (Imgenex #IMG-3049), Rabbit anti-Kif5C (Acris # SP5236P), Mouse anti-Phospho-Tyrosine (Cell Signaling Technology #9411). Fluorescent, Dylight conjugated secondary antibodies were purchased from Jackson Immunoresearch, and HRP conjugated secondary antibodies used for Western blot were from BioRad. Whole IgG negative controls for IP experiments were from Sino Biological (Goat IgG #CR2-500) and Millipore (Mouse IgG #12–371).

Free full text: Click here

Cheng H., Burroughs-Garcia J., Birkness J.E., Trinidad J.C, & Deans M.R. (2016). Disparate Regulatory Mechanisms Control Fat3 and P75NTR Protein Transport through a Conserved Kif5-Interaction Domain. PLoS ONE, 11(10), e0165519.

Publication 2016

Rabbit anti-dsRed Rat anti-E Cadherin Goat anti-GFP Rabbit anti-GST Mouse anti-HA Mouse anti-Phospho-Tyrosine Dylight conjugated secondary antibodies HRP conjugated secondary antibodies

Antibodies Biological Goat Kif5b Mouse Rabbit Rat e cadherin Sino Tyrosine Western blot

Top 5 similar protocols

Variable analysis

independent variables

Rabbit anti-dsRed (Clontech #632496)
Rat anti-E Cadherin (Life Technologies #13–1900)
Rabbit anti-Fat3 and polyclonal Mouse anti-Fat3 (Lisa Goodrich, Harvard Medical School [18 (link)])
Goat anti-GFP (Abcam #5450, IF and WB)
Goat anti-GFP (Abcam #6673, IP only)
Rabbit anti-GST (Cell Signaling Technology #2625S)
Mouse anti-HA (Covance #MMS-101P)
Mouse anti-Kif1A (BD Biosciences #612094)
Rabbit anti-Kif5B (Thermo # PA1-642)
Goat anti-Kif5B (Imgenex #IMG-3049)
Rabbit anti-Kif5C (Acris # SP5236P)
Mouse anti-Phospho-Tyrosine (Cell Signaling Technology #9411)
Fluorescent, Dylight conjugated secondary antibodies (from Jackson Immunoresearch)
HRP conjugated secondary antibodies (from BioRad)

dependent variables

Not explicitly mentioned

control variables

Whole IgG negative controls for IP experiments (from Sino Biological (Goat IgG #CR2-500) and Millipore (Mouse IgG #12–371))

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!