Immunohistochemical Detection of IDH1 R132H

The IHC clone used was anti-IDH1 R132H (Dianova, USA Clone: H09-unconj) in dilution of 1:25. Immunoreactions were scored positive for mutated IDH1 protein when a majority of tumor cells showed strong cytoplasmic positivity with or without nuclear staining.[16 (link)] Normal/residual glial cells and vascular endothelial cells served as an internal negative control.

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Anand N., Husain N., Varshney R., Malhotra K.P, & Kaif M. (2021). Molecular classification and stratification of adult diffuse gliomas: A tertiary care center study. Journal of Carcinogenesis, 20, 20.

Publication 2021

anti-IDH1 R132H

Clone Cytoplasmic Dilution Glial Mutated protein Normal cells Tumor Vascular endothelial cells

Corresponding Organization : Dr. Ram Manohar Lohia Institute of Medical Sciences

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Variable analysis

independent variables

Concentration of anti-IDH1 R132H antibody (1:25 dilution)

dependent variables

Immunoreactivity of mutated IDH1 protein in tumor cells (strong cytoplasmic positivity with or without nuclear staining)

control variables

Normal/residual glial cells and vascular endothelial cells (serve as internal negative control)

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