In this study, polymers based on HPCD linked by succinic anhydride were synthesized according to the procedure outlined in reference [15] (link). Briefly, a specified amount of HPCD solution (C = 60 mg/mL) was heated at 100 • C in the presence of NaH 2 PO 4 . Subsequently, the linker's aqueous solution (3 M succinic anhydride) was added dropwise to the reaction mixture, which was stirred for 1.5 h. The molar ratio of HPCD to linker was maintained at 1:15.
The obtained polymer, referred to as HPolS, was purified through dialysis using a membrane with a molecular weight cut-off of 12 kDa (Serva, Heidelberg, Germany). The purified polymer was then dried for 24 h at 37 • C.
The concentration of cyclodextrin (CD) torus in HPolS was determined using FTIR spectroscopy, specifically by analyzing the 1032 cm -1 band corresponding to the C-O-C bond in the CD torus. Calibration dependence was established for accurate concentration determination.
The obtained polymer, referred to as HPolS, was purified through dialysis using a membrane with a molecular weight cut-off of 12 kDa (Serva, Heidelberg, Germany). The purified polymer was then dried for 24 h at 37 • C.
The concentration of cyclodextrin (CD) torus in HPolS was determined using FTIR spectroscopy, specifically by analyzing the 1032 cm -1 band corresponding to the C-O-C bond in the CD torus. Calibration dependence was established for accurate concentration determination.
