Rapid RNA and DNA Extraction from Bacteria

Bacterial RNA was obtained utilizing the RNAsnap method (25 (link)). Briefly, 1ml of saturated culture was centrifuged and the cell pellet was stored at −80°C. The pellet was then suspended in 500 μl RNAsnap mix (95% formamide, 18 mM ethylenediaminetetraacetic acid, 0.025% sodium dodecyl sulphate and 1% B-mercaptoethanol), lysed using ∼200 μl of 0.1mm Zirconia Silica beads [Biospec 11079101Z] using a BioSpec mini bead beater [Biospec 1001] for 2 min in a deep well 96-well plate [Axygen P-DW-20-C]. The plate was centrifuged and the clarified lysate was cleaned up using a Zymo ZR-96 RNA Clean & Concentrator kit [Zymo R1080] per manufacturer's instructions. Bacterial DNA was obtained using our standard automated DNA extraction pipeline based on a scaled down version of the Qiagen MagAttract PowerMicrobiome DNA/RNA Kit [Qiagen 27500–4-EP], detailed fully in (26 (link)).