Immunohistochemical staining was performed as described in our previous work (Li et al., 2019 (link)), using the Mouse- and Rabbit-specific HRP/DAB (ABC) Detection IHC Kit (ab64264; Abcam, Cambridge, United Kingdom) following the manufacturer’s protocol. Briefly, the tissue sections were deparaffinized and rehydrated. Epitope retrieval was performed in ethylenediaminetetraacetic acid (EDTA). After incubation with primary antibody overnight, HRP conjugated secondary antibody was used. For immunohistochemical detection, tissue was subsequently counterstained with diaminobenzidine, hematoxylin and hydrated. It is replaced the primary antibody with PBS as negative controls. Staining intensity was evaluated by ImageJ-Pro Plus 6.0 software. Pictures were captured under a Leica DMi8 microscope (Wetzlar, Germany).
Immunofluorescence staining of tissues was performed as described previously (Zhang et al., 2018 (link)).
Immunofluorescence staining of tissues was performed as described previously (Zhang et al., 2018 (link)).
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