Human embryonic kidney (HEK)293T/17 cells (ATCC: CRL11268) stably expressing a SARS-CoV-2 entry receptor of human cells, hACE2 (HEK293T/17-hACE2) were generated by lentivirus transduction as described previously [36 (link),37 (link)] and maintained in Dulbecco’s Modified Eagle Medium/high glucose (Hyclone Laboratories, Logan, UT, USA) supplemented with 10% (v/v) fetal bovine serum and antibiotics [36 (link),38 (link)].
The HIV-based lentivirus carrying a firefly luciferase reporter gene was pseudotyped with SARS-CoV-2 S protein (SARS-CoV-2 S pseudotyped HIV) by the method mentioned previously [36 (link),39 (link)]. Briefly, the infectious viral particles were generated by co-transfection of pCSFLW, pCMV-ΔR8.91, and the pCAGGS plasmid carrying codon-optimized SARS-CoV-2 S gene (GenBank: YP_009724390.1) into HEK293T/17 cells. The supernatant containing SARS-CoV-2 S pseudotyped viral particles was harvested and stored at −80 °C until used.
The HIV-based lentivirus carrying a firefly luciferase reporter gene was pseudotyped with SARS-CoV-2 S protein (SARS-CoV-2 S pseudotyped HIV) by the method mentioned previously [36 (link),39 (link)]. Briefly, the infectious viral particles were generated by co-transfection of pCSFLW, pCMV-ΔR8.91, and the pCAGGS plasmid carrying codon-optimized SARS-CoV-2 S gene (GenBank: YP_009724390.1) into HEK293T/17 cells. The supernatant containing SARS-CoV-2 S pseudotyped viral particles was harvested and stored at −80 °C until used.
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