The genes ramR, marR, soxR and rpsJ were amplified and sequenced with the primers described previously [18 (link),21 (link)].
Standard PCR protocols and conditions were used in the following way: initial denaturation at 94 °C for 5 min; 35 cycles of 94 °C for 30 s, 52 °C for 1 min and 72 °C for 1 min; and a final incubation for 5 min at 72 °C. We used Taq DNA polymerase and dNTPs from QIAGEN (Hilden, Germany), and a T3000 Biometra thermocycler (Biometra, Germany).
Sequencing was performed with the Mix2Seq Kit (Eurofins Genomics).
Sequence analysis was performed with Serial Cloner v2.6 and BLAST (Basic Local Alignment Search Tool,https://blast.ncbi.nlm.nih.gov/Blast.cgi ).
Standard PCR protocols and conditions were used in the following way: initial denaturation at 94 °C for 5 min; 35 cycles of 94 °C for 30 s, 52 °C for 1 min and 72 °C for 1 min; and a final incubation for 5 min at 72 °C. We used Taq DNA polymerase and dNTPs from QIAGEN (Hilden, Germany), and a T3000 Biometra thermocycler (Biometra, Germany).
Sequencing was performed with the Mix2Seq Kit (Eurofins Genomics).
Sequence analysis was performed with Serial Cloner v2.6 and BLAST (Basic Local Alignment Search Tool,
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