BAC Probe Hybridization in Telomeric Regions

BAC (bacterial artificial chromosome) probes were hybridized on the same slides according to Mary et al. (2014) [8 (link)]. These BAC probes were selected in the telomeric regions of the chromosomes [27 (link)] and were obtained from the Biological Resources Center-GADIE (http://www-crb.jouy.inra.fr/) [28 (link)].
Different combinations of probes were used:
Probes were labeled with biotin or digoxygenin using the bioprime labeling system (Invitrogen) and revealed using Alexa 594 conjugated to Streptavidin (Molecular Probes, Eugene, OR, USA) and FITC conjugated mouse anti-digoxygenin antibodies (Sigma, St Louis, MO).

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Mary N., Barasc H., Ferchaud S., Priet A., Calgaro A., Loustau-Dudez A.M., Bonnet N., Yerle M., Ducos A, & Pinton A. (2016). Meiotic Recombination Analyses in Pigs Carrying Different Balanced Structural Chromosomal Rearrangements. PLoS ONE, 11(4), e0154635.

Publication 2016

bioprime labeling system Alexa 594 conjugated to Streptavidin FITC conjugated mouse anti-digoxygenin antibodies

Alexa 594 Anti antibodies Bacterial artificial chromosome Biological Biotin Chromosomes Fitc Molecular probes Mouse Streptavidin Telomeric

Corresponding Organization : École Nationale Vétérinaire de Toulouse

Other organizations : Département Génétique Animale

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Variable analysis

independent variables

BAC (bacterial artificial chromosome) probes

dependent variables

Hybridization pattern of BAC probes

control variables

Slides used for BAC probe hybridization according to the protocol in Mary et al. (2014)
BAC probes selected in the telomeric regions of the chromosomes
BAC probes obtained from the Biological Resources Center-GADIE

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

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