Humanized mice were infected at 20 weeks of age with HIV NLEG1-70 GFP+ virus via intraperitoneal (i.p.) injection at a single dose of 105 TCID50/ml. At 4 weeks and 10 weeks post-infection (w.p.i.), levels of viral RNA copies/ml were analyzed with the automated COBAS Ampliprep system V2 (Roche Molecular Diagnostics, Mannheim, Germany) as described19 (link).
Free full text: Click here