Evaluating Endothelial Progenitor Cells

Endothelial progenitor cell-colony forming units were used to assess endothelial function and assays were performed as previously described (Premer et al., 2015 (link)). Briefly, EPC-CFUs were determined by isolating EPCs from peripheral blood samples, plating them on 6-well fibronectin-coated dishes followed by 24-well fibronectin-coated dishes in CFU-Hill medium (Stem Cell Technologies, cat #05900), and counting EPC-CFU formation on day five.

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Premer C., Wanschel A., Porras V., Balkan W., Legendre-Hyldig T., Saltzman R.G., Dong C., Schulman I.H, & Hare J.M. (2019). Mesenchymal Stem Cell Secretion of SDF-1α Modulates Endothelial Function in Dilated Cardiomyopathy. Frontiers in Physiology, 10, 1182.

Publication 2019

CFU-Hill medium

Assays Blood Dishes Endothelial Endothelial progenitor cell Fibronectin Stem cell

Corresponding Organization : University of Miami

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Variable analysis

independent variables

Endothelial progenitor cell-colony forming units (EPC-CFUs)

dependent variables

Endothelial function

control variables

Peripheral blood samples
6-well fibronectin-coated dishes
24-well fibronectin-coated dishes
CFU-Hill medium (Stem Cell Technologies, cat #05900)

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

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