Culturing A549 cells with VIM-RFP

A549/VIM-RFP cells were obtained from (ATCC CCL-185EMT, RRID:CVCL_LI35), and cells within 5–15 generations were used in the studies. Cells were cultured in F-12K medium (Corning) with 10% fetal bovine serum (FBS) in MatTek glass bottom culture dishes (P35G-0–10 C) in a humidified atmosphere at 37 °C and 5% CO2, as detailed in Wang et al., 2020 (link). The cell line was authenticated as A549 cells with short tandem repeat (STR) analysis by University of Arizona Genetics Core (UAGC). The result of mycoplasma contamination test (MycoFluor Mycoplasma Detection Kit Molecular Probes, M-7006) was negative.

Free full text: Click here

Wang W., Poe D., Yang Y., Hyatt T, & Xing J. (2022). Epithelial-to-mesenchymal transition proceeds through directional destabilization of multidimensional attractor. eLife, 11, e74866.

Publication 2022

CCL-185EMT F-12K medium MycoFluor Mycoplasma Detection Kit

A549 cells Atmosphere Cell line Cells Cultured medium Fetal bovine serum Molecular probes Mycoplasma Short tandem repeat

Corresponding Organization :

Other organizations : University of Pittsburgh, Carnegie Mellon University, UPMC Hillman Cancer Center

Top 5 similar protocols

Variable analysis

independent variables

Cell line (A549/VIM-RFP cells)

dependent variables

Not explicitly mentioned

control variables

Cell culture conditions (F-12K medium with 10% FBS, 37 °C, 5% CO2)
Cell passage number (5-15 generations)
Cell culture vessel (MatTek glass bottom culture dishes)

controls

Positive control: Not specified
Negative control: Not specified

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!