Human iPSC Lines for ALS Research

Human iPSC lines were obtained from the Target ALS Repository, Harvard University, Jackson Laboratory, and the NINDS Human Cell and Data Repository (NHCDR). Human iPSC lines included non-isogenic control lines 11a [9 (link)], ND50003 (FA0000010) and KOLF2.1 J [75 (link)] and five fALS lines harboring mutations in C9orf72: 19f [45 (link)], NDS00268 (ND50074), NDS00269 (ND50075), NDS00270 (ND50076), and NDS00273 (ND50080). An isogenic pair of lines consisting of an unedited control TDP-43^+/+ line and an edited TDP-43^+/G298S iPSC line (harboring a single fALS mutation) was previously generated [77 (link)]. iPSCs were cultured as described previously [65 (link)]. Briefly, iPSCs were maintained on Matrigel-coated 6 well-plates (Corning, 354,277, and Falcon, 353,046) in mTeSR media (Stemcell Technologies, 85,850) for SMN differentiation or mTeSR Plus media (Stemcell Technologies, 100–0274) for NGN2 induction at 37 °C with 5% CO₂.