Murine Renal Xenograft Protocol

Wild type C57BL6/N (C57BL/6NTac, 8–10 weeks old) and Swiss-Webster (8–10 weeks) mice were purchased from Taconic. The UBC-GFP (C57BL/6-Tg(UBC-GFP)30Scha/J, 8–13 weeks old; JAX #004353)(Schaefer et al., 2001 (link)) and R26R–YFP (B6.Cg–Gt(ROSA)26Sor^tm3^{(CAG−EYFP)Hze}/J; JAX #007903) (Madisen et al., 2010 (link)) mice were obtained from the Jackson Laboratory. The Nkx3-1^Cre allele has been previously described (Lin et al., 2007 (link); Thomsen et al., 2008 (link)). As hosts for renal grafts, R2G2 mice (B6;129- Rag2^tm1FwaIl2rg^tm1Rsky/DwIHsd, 8–15 weeks old) were purchased from Envigo, and NOD/SCID mice (NOD.Cg-Prkdc^scid/J, 8–14 weeks old; JAX #001303) were purchased from the Jackson Laboratory. To obtain urogenital mesenchyme for tissue recombination and renal grafting, we used E18 Sprague-Dawley rat embryos from timed matings (Charles River #400). Animal studies were approved by and conducted according to standards set by the Columbia University Irving Medical Center (CUIMC) Institutional Animal Care and Use Committee (IACUC).

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Crowley L., Cambuli F., Aparicio L., Shibata M., Robinson B.D., Xuan S., Li W., Hibshoosh H., Loda M., Rabadan R, & Shen M.M. (2020). A single-cell atlas of the mouse and human prostate reveals heterogeneity and conservation of epithelial progenitors. eLife, 9, e59465.

Publication 2020

Swiss-Webster UBC-GFP (C57BL/6-Tg(UBC-GFP)30Scha/J R26R–YFP (B6.Cg–Gt(ROSA)26Sortm3(CAG−EYFP)Hze/J; R2G2 mice (B6;129- Rag2tm1FwaIl2rgtm1Rsky/DwIHsd, NOD/SCID mice (NOD.Cg-Prkdcscid/J JAX #001303 E18 Sprague-Dawley rat embryos

Allele Animal Embryos Iacuc Mesenchyme Mice Nod mice Recombination River Rosa Scid mice Sprague dawley rat Tissue Urogenital

Corresponding Organization :

Other organizations : Columbia University Irving Medical Center, Cornell University

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Variable analysis

independent variables

Mouse strains: Wild type C57BL6/N (C57BL/6NTac, 8–10 weeks old), Swiss-Webster (8–10 weeks), UBC-GFP (C57BL/6-Tg(UBC-GFP)30Scha/J, 8–13 weeks old), R26R–YFP (B6.Cg–Gt(ROSA)26Sortm3(CAG−EYFP)Hze/J, 8–15 weeks old), Nkx3-1Cre allele, R2G2 mice (B6;129-Rag2tm1FwaIl2rgtm1Rsky/DwIHsd, 8–15 weeks old), NOD/SCID mice (NOD.Cg-Prkdcscid/J, 8–14 weeks old), and E18 Sprague-Dawley rat embryos
Age (8–10 weeks, 8–13 weeks, 8–15 weeks, 8–14 weeks)

dependent variables

Not explicitly mentioned

control variables

Sex (not specified)
Housing conditions (not specified)
Environmental factors (not specified)

positive controls

None specified

negative controls

None specified

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