Nintedanib Modulates Signaling Pathways in Triple-Negative Breast Cancer

MDA-MB-231, MDA-MB-468 and HCC-1395 cell lines were obtained from the ATCC (American Type Culture Collection, Rockville, MD, USA) and maintained in Dulbecco’s Modified Eagle Medium (DMEM, Gibco, Carlsbad, CA, USA) supplemented with 10% fetal bovine serum (FBS, HyClone, Thermo Scientific, Waltham, MA, USA). Cell lysates treated with nintedanib were prepared and analyzed by western blot as previously reported.^{18 (link)} Antibodies targeting p-JAK2 (Tyr1007/1008), JAK2, p-SRC (Tyr416), SRC, p-STAT3 (Tyr705), STAT3, survivin, poly (ADP-ribose) polymerase (PARP) and cleaved caspase 3 were purchased from Cell Signaling (Danvers, MA, USA). SHP-1, cyclin D1 and Mcl-1 antibodies were purchased from Abcam (Cambridge, MA, USA).