HEK293T (RRID:CVCL_0063), JHH7 (RRID:CVCL_2805), and SNU449 AXIN1-repaired cells (clone 20, RRID:CVCL_0454) were cultured in DMEM (Gibco) supplemented with 10% (v/v) FBS (Gibco). SNU449 AXIN1-repaired cells were generated using CRISPR-Cas9 genome editing, as previously described (12 (link)). Cells were cultured in a humidified incubator maintained at 37°C with 5% CO2. All cell lines tested negative for Mycoplasma based on the real-time PCR method at Eurofins GATC-Biotech. Identity of all cell lines and clones thereof was confirmed by the Erasmus Molecular Diagnostics Department using Powerplex-16 STR genotyping (Promega).
For the preparation of conditioned medium, L-Control [L-M(TK-), RRID:CVCL_4536] and L-Wnt3A (RRID:CVCL_0635) cells were cultured in complete DMEM medium, followed by collection and filtration of medium according to the standard procedures.
For the preparation of conditioned medium, L-Control [L-M(TK-), RRID:CVCL_4536] and L-Wnt3A (RRID:CVCL_0635) cells were cultured in complete DMEM medium, followed by collection and filtration of medium according to the standard procedures.
