SureSelect target capture was used as previously described [12 (link)] on a selection of 95 isolates including selected samples from a previous study on LGV diversity [19 ]. The SureSelect baits were custom designed around known variable regions from all sequenced ompA-genotypes [9 (link)]. Sequence capture (48-plex) was performed according to the manufacturer’s protocols (Agilent). After library preparation [34 (link)], multiplexed (96-plex) samples were sequenced using Illumina Hiseq V4 (Illumina) with 75 bp paired-end reads.
Protocol detail
Targeted Sequencing of Chlamydia ompA Genotypes
Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link:
Access Free Full Text.
Genotypes
Library
Corresponding Organization :
Other organizations : University Hospital of Basel, University of Basel, SIB Swiss Institute of Bioinformatics, Universitat Autònoma de Barcelona, Wellcome Sanger Institute, University of Amsterdam, Amsterdam University Medical Centers, Public Health Service of Amsterdam, London School of Hygiene & Tropical Medicine, Uppsala University, Westmead Hospital, University of Sydney, Institut Polytechnique de Bordeaux, Sydney Sexual Health Centre, Sydney Local Health District, Instituto de Investigación Sanitaria del Hospital Clínico San Carlos, University of Ljubljana, National Center for Epidemiology, National Institute of Public Health, Charles University, Maastricht University, Amsterdam UMC Location VUmc, Instituto Ramón y Cajal de Investigación Sanitaria, Centro de Investigación Biomédica en Red de Epidemiología y Salud Pública, Hospital Universitario Ramón y Cajal
Top 5 similar protocols
Variable analysis
independent variables
- Selection of 95 isolates including selected samples from a previous study on LGV diversity
- Sequenced ompA-genotypes
- Known variable regions from all sequenced ompA-genotypes
- Positive control: Not specified
- Negative control: Not specified
Annotations
Based on most similar protocols
Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.
Sign up for free or login to display all annotations
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!