Fresh leaves, young stems, and flowers were collected from a 500-year-old C. camphora in Wuxue (115.56 E, 29.84 N), Hubei Province, China. High-quality genomic DNA was extracted from young leaves using a DNeasy Plant Mini Kit (Qiagen, Germany). The concentration and purity of the extracted DNA were assessed using a Nanodrop 2000 spectrophotometer (Thermo, MA, USA) and Qubit 3.0 (Thermo, CA, USA). The integrity of the DNA was determined using pulsed-field electrophoresis with a 0.8% agarose gel.
For genome sequencing, approximately 20-kb SMRTbell libraries were constructed for long-read sequencing on the PacBio Sequel platform at the Biomarker Technologies Corporation, Beijing. Additionally, short-read libraries were constructed and sequenced on an Illumina X-ten platform (Illumina, CA, USA) with 150-bp paired-end reads. After quality control, 111.06 Gb of PacBio single-molecule long reads and 49.59 Gb Illumina paired-end short reads were generated and used for assembly evaluation and genome correction. RaGOO [27 (link)] was used to anchor contigs and scaffolds to the C. kanehirae reference chromosome. A Hi-C library from young leaves was constructed by the Illumina Nova platform to verify the results of chromosome allocation.
For genome sequencing, approximately 20-kb SMRTbell libraries were constructed for long-read sequencing on the PacBio Sequel platform at the Biomarker Technologies Corporation, Beijing. Additionally, short-read libraries were constructed and sequenced on an Illumina X-ten platform (Illumina, CA, USA) with 150-bp paired-end reads. After quality control, 111.06 Gb of PacBio single-molecule long reads and 49.59 Gb Illumina paired-end short reads were generated and used for assembly evaluation and genome correction. RaGOO [27 (link)] was used to anchor contigs and scaffolds to the C. kanehirae reference chromosome. A Hi-C library from young leaves was constructed by the Illumina Nova platform to verify the results of chromosome allocation.
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