To propagate the recombinant baculoviruses and titrate them we used the Spodoptera frugiperda cell line, Sf9 (ATCC®, USA, cat. no. CRL-1711). Cells were maintained in Grace’s medium (Thermo Fisher, USA, cat. no. 11300–027) supplemented with 10% inactivated fetal bovine serum (FBS) (Biowest, France, cat. no. S1650–500), lactoalbumin (Sigma-Aldrich, USA, cat. no. 19010), yeastolate (Thermo Fisher, USA, cat. no. 292805), antibiotic-antimycotic (Thermo Fisher, USA, cat. no. 15240–062) and 0.1% pluronic acid F- 68 (Sigma-Aldrich, USA, cat. no. P1300) at 27 °C under agitation, as previously described [28 (link)].
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