Genetic Models of Myeloid HDAC7 Regulation

Male and female C57BL/6J mice of 8 to 12 wk of age were obtained from an in-house breeding colony within the QBP animal house (The University of Queensland) or from the animal resources centre (Murdoch, WA). Mac-Hdac7 mice, which overexpress HDAC7 in myeloid cells, have previously been described (11 (link)). Littermate MacBlue mice expressing cyan fluorescent protein (CFP) in myeloid cells (79 (link)) were used as controls for studies using Mac-Hdac7 mice. Myeloid-deleted Hdac7 mice (Hdac7^−/−) (11 (link)) were obtained by crossing Hdac7^flox/flox/LysM^Cre mice with Hdac7^flox/flox mice, with littermate Hdac7^flox/flox (Hdac7^+/+) mice used as controls (80 (link)). As an additional control to ensure that the LysM^Cre allele did not confound data interpretation, functional assays (IL-1β release by ELISA, Escherichia coli-induced ROS production, and intracellular UPEC loads in macrophages) were performed to compare the phenotypes of BMM from LysM^Cre, Hdac7^+/+, and Hdac7^−/− mice (SI Appendix, Fig. S9 A–D). Tlr4^−/− mice (81 (link)) were maintained on a C57BL/6J background at the QBP animal house.