Plasma Biomarker Measurement Protocol

Plasma levels of alanine transaminase (ALT) and aspartate transaminase (AST) were determined using kinetic absorbance assays (Teco Diagnostics) as described previously [16 (link)]. Analysis of plasma triglycerides (Thermo Fisher Scientific), total cholesterol (Thermo Fisher Scientific), and non-esterified fatty acids (FUJIFILM Wako) were determined using colorimetric based assay as previously reported [50 (link), 51 (link)].

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Habibi M., Ferguson D., Eichler S.J., Chan M.M., LaPoint A., Shew T.M., He M., Lutkewitte A.J., Schilling J.D., Cho K.Y., Patti G.J, & Finck B.N. (2023). Mitochondrial Pyruvate Carrier Inhibition Attenuates Hepatic Stellate Cell Activation and Liver Injury in a Mouse Model of Metabolic Dysfunction-associated Steatotic Liver Disease. bioRxiv.

Publication Preprint 2023

total cholesterol non-esterified fatty acids

Alanine transaminase Aspartate transaminase Assay Cholesterol Colorimetric Diagnostics Esterified fatty acids Kinetic assays Plasma Triglycerides

Corresponding Organization : Washington University in St. Louis

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Plasma levels of alanine transaminase (ALT)
Plasma levels of aspartate transaminase (AST)
Plasma triglycerides
Total plasma cholesterol
Non-esterified fatty acids (NEFA)

control variables

None explicitly mentioned

controls

No positive or negative controls were explicitly mentioned in the input.

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